Fatty Acid Selectivity of Lipases during Acidolysis Reaction between Triolein and Saturated Fatty Acids Varying from Caproic to Behenic Acids

KARABULUT İ., Durmaz G., HAYALOĞLU A. A.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, cilt.57, sa.16, ss.7584-7590, 2009 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 57 Sayı: 16
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1021/jf901755h
  • Dergi Adı: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.7584-7590
  • Anahtar Kelimeler: Lipase, selectivity, acidolysis, saturated fatty acid, triolein, Thermomyces lanoginosus, Rhizomucor miehei, Candida antarctica, PRODUCE STRUCTURED LIPIDS, CAPRYLIC-ACID, CATALYZED INTERESTERIFICATION, ENZYMATIC MODIFICATION, GEOTRICHUM-CANDIDUM, CHAIN-LENGTH, BINDING SITE, TRIACYLGLYCEROLS, ESTERIFICATION, HYDROLYSIS
  • İnönü Üniversitesi Adresli: Evet

Özet

The chain length selectivity of three immobilized lipases, namely, Lipozyme TL IM from Thermomyces lanoginosus, Lipozyme RM IM from Rhizomucor miehei, and Novozym 435 from Candida antarctica, was determined in acidolysis performed in hexane using the homologous series of even carbon number, saturated fatty acids (SFAs) of 6-22 carbons. Triolein with individual SFAs or a mixture of equimolar quantities of SFAs was used as the substrate. The effects of operating variables including the mole ratio of fatty acid to triolein, temperature, enzyme dosage, and time on incorporation were also investigated. Incorporation abilities of the enzymes tested were found to be significantly different for most of FAs at the experimental conditions evaluated. Lipases acted weakly on SFAs of which the carbon chain length was shorter than eight carbon atoms and higher than 18 carbon atoms. Lipases showed a bell-shaped distribution in incorporation vs chain length plot with a maximum around C12-C16. Among the experimental parameters tested, the effect of the substrate mole ratio was greater than those of the others, and the highest incorporation was observed for C12 (36.98%), C14 (37.63%), and C16 (38.66%) at a 4:1 substrate mole ratio with Lipozyme TL IM. Lipases caused significantly different levels of acyl migration from sn-1,3 to sn-2 positions.