A new aromatic organosoluble polyimide was synthesized from the 4,4'-Diaminodiphenileethane with 3,3',4,4'-benzophenonetetracarboxylic dianhydride (BTDA) via a conventional two-stage process. This polymer showed good solubilities in N-methyl-2-pyrrolidone and m-cresol. The polyimide was characterized by FT-IR, GPC, TGA, DSC and DTA, with intrinsic viscosity and adhesive properties also being evaluated. Then, glucose oxidase (GOD) enzyme was immobilized into aromatic organosoluble polyimide by two different methods. It was demonstrated that the poly[4,4'-Diamina very promising substrate for the immobilization and stabilization of enzymes and the development of highly stable biosensors.