Poly(3-methylthiophene) modified gold sensor for determination of 3,4-dihydroxyphenylacetic acid, ascorbic acid and uric acid in biological fluids using voltammetric techniques


KUYUMCU SAVAN E. , ERDOĞDU G.

JOURNAL OF RESEARCH IN PHARMACY, cilt.23, ss.25-33, 2019 (ESCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 23 Konu: 1
  • Basım Tarihi: 2019
  • Doi Numarası: 10.12991/jrp.2018.105
  • Dergi Adı: JOURNAL OF RESEARCH IN PHARMACY
  • Sayfa Sayıları: ss.25-33

Özet

Electrochemical polymerization was carried out in a one-compartment cell containing deaerated acetonitrile, and 100 mM tetrabutylammonium tetrafluoroborate monomer of 3-methylthiophene. The polymer films were grown on a gold electrode by bulk electrolysis at 1.700 V (vs. Ag/Ag+). The modified sensor showed an excellent electrocatalytic effect towards oxidation of the mixture of 3,4-dihydroxyphenylacetic acid (DOPAC), ascorbic acid and uric acid, which appeared at a pH range of 2-11 by differential pulse voltammetry (DPV) techniques. The best results for determination of DOPAC were obtained by DPV in PBS (pH 7.00). A calibration curve was obtained in the DOPAC concentration range of 1.0-50.0 mu M (r = 0.9948). Limit of detection and limit of quantification were found as 0.066 mu M and 0.220 mu M, respectively. The results of the experiments indicated that the sensor has good stability, sensitivity and reproducibility for at least one month if stored in dry air.