Akademik Veri Yönetim Sistemi
5th Eurasia Biochemical Approaches & Technologies (EBAT), Antalya, Türkiye, 2 - 05 Kasım 2023, ss.2
Rapid and accurate detection of viral pathogens is crucial for
effective disease management and epidemic control. The pandemic also has
necessitated the development of scalable diagnostic methods for the detection
of viruses.
Aptamers, which are short, single-stranded nucleic acid molecules,
enable the identification of viral biomarkers by exhibiting high affinity and
specificity for their targets. Easy modification processes enable
custom-designed binding features, improving test performance. Their reduced
size facilitates better penetration of complex biological samples while minimizing
nonspecific binding.
In this study, it was aimed to optimize the aptamer usage for
preparation of the point of care test for viral detection. For this purpose,
gold nanoparticles (AuNPs) were synthesized and used as label. Aptamer was
modified with –SH (thiol) bond before the conjugation of AuNPs and also
modified with biotin for surface immobilization. Then, different concentrations
of aptamer were adopted to conjugation for the same purpose.
Results showed that using aptamers for labelling the AuNPs has
more advantages compared to the antibody labelling in terms of the efficiancy,
time, shelf life and stability for preparing the LFAs. In short, this work
summarizes the distinct advantages offered by the use of aptamers in LFAs
specifically designed for viral detection compared to antibody.