Akademik Veri Yönetim Sistemi
World Journal of Microbiology and Biotechnology, cilt.41, sa.10, 2025 (SCI-Expanded, Scopus)
Recently, focus has been placed on various enzyme immobilization applications as effective, eco-friendly, and low-cost methods for the degradation of pesticides in water. Laccases are very effective biocatalysts that are frequently used in environmental remediation applications. In this research, the use of a biocatalyst obtained by immobilizing laccase purified from Trametes trogii on chitosan-polyethylene glycol methyl ether (Lac/CH/MPEG) in the degradation of atrazine (ATR) and trifloxystrobin (TFS) pesticides in water was investigated. The degradation of these pesticides with this synthesized biocatalyst is the first in the literature. Therefore, primarily, the basic physicochemical parameters and mechanical properties of Lac/CH/MPEG prepared before and after immobilization were investigated. Structural analysis and morphological imaging of Lac/CH/MPEG before and after degradation of ATR and TFS were also performed. In the degradation tests, the initial concentrations at which optimum degradation rates were observed for ATR (72%) and TFS (85%) were 1 mg L−1 and 0.5 mg L−1, and the contact times were 24 h and 12 h, respectively. Additionally, the reuse cycles of Lac/CH/MPEG in ATR and TFS degradation were found to be 5 and 6, respectively. These findings showed that TFS was degraded faster and more effectively than ATR. Compared to several biocatalysts in the literature, ours was shown to degrade pesticides more quickly and efficiently. Considering all the findings, it can be concluded that this novel Lac/CH/MPEG biocatalysts is promising, can be applied, and can be successfully used as an alternative agent for quick and efficient degradation of ATR and TFS in water/wastewater.