SOUTH AFRICAN JOURNAL OF BOTANY, cilt.141, ss.177-182, 2021 (SCI-Expanded)
Salvia palaestina aqueous and methanol extracts were prepared from the aerial parts, which were evaluated
for the in vitro anti-inflammatory properties using the lipoxygenase (LO) enzyme inhibition assay. While the
aqueous extract showed no activity at test concentrations, a significant (p < 0.001) lipoxygenase inhibition
was detected for the methanol extract with 29% inhibition. Activity guided fractionation was carried out on
the methanol extract via liquid-liquid partitioning using n-hexane, dichloromethane, ethyl acetate, and nbutanol. The ethyl acetate fraction showed statistically the best inhibition among the sub-fractions with 70%
inhibition (p < 0.0001). The compounds responsible for the activity were purified, and their structures were
established as rosmarinic acid, and methyl rosmarinate by spectroscopic methods. IC50 values of rosmarinic
acid, and methyl rosmarinate were calculated as 0.21 and 0.02 mM, respectively. In conclusion, the in vitro
anti-inflammatory potential of S. palaestina was associated to rosmarinic acid, and methyl rosmarinate, for
the first time to the best of our knowledge.