Synthesis, characterization, and investigation of antiproliferative activity of novel Ag (I)-N-Heterocyclic Carbene (NHC) compounds


Cevik-Yildiz E., ŞAHİN N., ŞAHİN BÖLÜKBAŞI S.

JOURNAL OF MOLECULAR STRUCTURE, cilt.1199, 2020 (SCI İndekslerine Giren Dergi) identifier identifier

Özet

The aim of this study was to present the synthesis, characterization, and investigation of the antiproliferative activity of new metal N-Heterocyclic Carbene (NHC) salts (1a-c) and their Ag(I) complexes (2a-c). All synthesized compounds were characterized using elemental analysis, LC-MS, FT-IR,H- 1 NMR, and C-13 NMR spectroscopy techniques. Salts and complexes were tested for antiproliferative activities on human breast and prostate cancer cell lines (MCF-7, MDA-MB-23, DU-145) and L-929 normal cells for 24 h, 48 h and 72 h using MTT assays. The Ag(I)-NHC complexes (2a c) showed dose and time-dependent cytotoxic activity against all cell lines. MDA-MB-231 and MCF-7 human breast carcinoma cells were the most sensitive to displaying IC50 lower than 1 mu M at all time points for 2a and 2b complexes respectively. The IC(50)s for Ag(I)-NHC were higher in normal cells especially compared to the breast cancer cells, suggesting that complexes possessed noteworthy selectivity for human breast cancer cells. Complex 2a showed high selectivity (>= 13-fold) for MDA-MB-231 breast cancer cells at all time points. These results also demonstrated that complex 2b has 4-7-fold selectivity against MCF-7 breast cancer cells. (C) 2019 Published by Elsevier B.V.