The preparation and sensor application of poly(p-aminophenol)

Ekinci E. , Karagozler A., Karagozler A.

ELECTROANALYSIS, cilt.8, ss.571-574, 1996 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 8 Konu: 6
  • Basım Tarihi: 1996
  • Doi Numarası: 10.1002/elan.1140080613
  • Sayfa Sayıları: ss.571-574


An amperometric biosensor for glucose based on a novel polymeric matrix is described. The sensor was prepared by electrochemical polymerization of p-aminophenol in the presence of glucose oxidase on Pt substrates in a KCl solution at a potential of 0.6 V (vs. Ag/AgCl). The chronoamperometric response of poly(p-aminophenol)-glucose oxidase biosensor to hydrogen peroxide formed as the result of enzymatic reaction was measured at a potential of 0.7 V (vs. Ag/AgCl) in PBS solution. It was shown that a biosensor prepared by this onestep procedure has a linear range up to 6 mM glucose, a response time less than 5 s and a lifetime around 15 days. The sensitivity of the electrode was superior to that of much publicized polypyrrole based glucose oxidase electrode. Also, the substrate selectivity of PAP/GOx electrode was tested for coexisting substances such as lactose, sucrose, urea, paracetamol, uric and ascorbic acid and no discernible anodic response was observed above the background current.