Fabrication of Oleic Acid Grafted Starch-based Hybrid Carriers for l-Asparaginase Encapsulation

Hafizosmanoglu G., Ulu A., KÖYTEPE S., ATEŞ B.

STARCH-STARKE, 2021 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1002/star.202100152
  • Dergi Adı: STARCH-STARKE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, Analytical Abstracts, BIOSIS, CAB Abstracts, Chemical Abstracts Core, Compendex, Food Science & Technology Abstracts, Veterinary Science Database
  • Anahtar Kelimeler: artificial red blood cells, encapsulation, enzyme immobilization, l-asparaginase, starch, ENZYME IMMOBILIZATION, CATALYTIC-ACTIVITY, PERFORMANCE, SUPPORT
  • İnönü Üniversitesi Adresli: Evet

Özet

In this study, artificial red blood cells (RBCs) for immobilization of l-asparaginase are developed. The artificial red blood cells are prepared via a self-assembly method and characterized by various methods. The prepared material shows a small hydrodynamic size of nearly 120 nm and a zeta potential of -7.2 +/- 0.2 mV. After l-asparaginase encapsulation, the particle size is nearly 295 nm and the zeta potential value is -9.8 +/- 0.1. As expected, the immobilized enzyme shows better pH stability and thermal stability than the free enzyme. For instance, at 60 degrees C, the immobilized enzyme maintains 63.5% of the original activity, while the free enzyme retains only 27.6% of the original activity. Furthermore, the immobilized enzyme exhibits excellent reusability and storage stability, retaining 46.1% of its initial activity after 15 reuses. Overall, the developed artificial RBCs can potentially be applied as a novel support for immobilization of l-asparaginase.