In this study, semen seminal plasma contents and the motility of sperm cells were determined in Capoeta trutta via a computer-assisted sperm analysis system. In addition, we evaluated the relationship between semen seminal plasma ions and the velocities of sperm cells. Although the predominant ions were K (206.84 +/- 20.61 mg L-1) and Na (128.06 +/- 23.82 mg L-1) in the semen seminal plasma, Ca (14.05 +/- 4.13 mg L-1) and Mg (3.35 +/- 0.44 mg L-1) were not predominate according to our results. However, partially strong relationships between the curvilinear velocity value (VCL) and K (R-2 = 0.67; p <0.05) were found, while it was moderate with Mg (R-2 = 0.48; p <0.05). There was a weak relationship with Na (R-2 = 0.17; p <0.05) and Ca (R-2 = 0.34; p <0.05). In our results, while the trace metals were determined as Zn > Al > B > Li > Cu in semen seminal plasma, they are not correlated with sperm cell velocities. Finally, we hope that the present information on the motility parameters of Capoeta trutta in this paper will eventually help artificial insemination in reproduction practices.