Investigating the possible protective effect of caffeic acid phenethyl ester on aquaporin-2 changes in renal ischemia-reperfusion injury in rats Investigación del posible efecto protector del éster fenetílico del ácido cafeico sobre los cambios de acuaporina-2 en la lesión por isquemia-reperfusión renal en ratas

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Sapmaz H. I., Köse E., Karaca Z. M., Akyürek S., Parlakpinar H., Deresoy F. A., ...Daha Fazla

Cirugia y Cirujanos, cilt.93, sa.4, ss.341-350, 2025 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 93 Sayı: 4
  • Basım Tarihi: 2025
  • Doi Numarası: 10.24875/ciru.24000295
  • Dergi Adı: Cirugia y Cirujanos
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE, Directory of Open Access Journals
  • Sayfa Sayıları: ss.341-350
  • Anahtar Kelimeler: Antioxidant, Aquaporin-2, Caffeic acid, Kidney, Phytochemical, Renal ischemia
  • İnönü Üniversitesi Adresli: Evet

Özet

Objective: We aimed to investigate the changes in renal aquaporins (AQP) of rats in renal ischemia-reperfusion (I/R) injury and the protective effects of caffeic acid phenethyl ester (CAPE) against these changes. Methods: Forty-five adult rats were divided into six groups: control, sham (right nephrectomy), I/R (right nephrectomy + left kidney I/R), I/R+CAPE (I/R procedure after i.p. CAPE), sham + CAPE, and sham + dimethyl sulfoxide. Blood urea nitrogen, Cr, and K+ levels were measured in the sera. Tissues were stained with hematoxylin and eosin for histopathological examination. For immunohistochemical analysis, AQP2 was applied using the streptavidin/biotin/peroxidase system. AQP2 gene expression in kidney tissues was examined by polymerase chain reaction (PCR). Results: In the I/R, congestion, inflammation, and necrosis were found to increase compared to the control. In the I/R+CAPE, improvement was observed in necrosis compared to the I/R. There was a decrease in AQP2 expression in the I/R. In PCR, no significant difference was observed in AQP2 gene expression between the I/R and control and between the I/R+CAPE and I/R. Conclusion: Renal I/R inhibits the production of AQP2 in the kidney and causes histological and biochemical damage. CAPE administration before I/R has a protective effect on the kidney.