Ascorbic acid and beta-carotene reduce stress-induced oxidative organ damage in rats.


Esrefoglu M., Akinci A., Taslidere E., Elbe H., Cetin A., Ates B.

Biotechnic & histochemistry : official publication of the Biological Stain Commission, vol.91, no.7, pp.455-464, 2016 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 91 Issue: 7
  • Publication Date: 2016
  • Doi Number: 10.1080/10520295.2016.1220019
  • Journal Name: Biotechnic & histochemistry : official publication of the Biological Stain Commission
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.455-464
  • Keywords: ascorbic acid, -carotene, microscopy, oxidative stress, rats, CHRONIC COLD-EXPOSURE, LIPID-PEROXIDATION, VITAMIN-C, IMMOBILIZATION STRESS, SUPEROXIDE-DISMUTASE, ANTIOXIDANT DEFENSE, PROTEIN OXIDATION, PROTECTIVE ROLE, BRAIN, GLUTATHIONE
  • Inonu University Affiliated: Yes

Abstract

Antioxidants are potential therapeutic agents for reducing stress-induced organ damage. We investigated the effects of ascorbic acid and -carotene on oxidative stress-induced cerebral, cerebellar, cardiac and hepatic damage using microscopy and biochemistry. Male Wistar albino rats were divided into five groups: untreated control, stressed, stressed + saline, stressed + ascorbic acid and stressed + -carotene. The rats in the stressed groups were subjected to starvation, immobilization and cold. The histopathological damage scores for the stressed and stressed + saline groups were higher than those of the control group for all organs examined. The histopathological damage scores and mean tissue malondialdehyde levels for the groups treated with antioxidants were lower than those for the stressed and stressed + saline groups. Mean tissue superoxide dismutase activities for groups that received antioxidants were higher than those for the stressed + saline group for most organs evaluated. Ascorbic acid and -carotene can reduce stress-induced organ damage by both inhibiting lipid oxidation and supporting the cellular antioxidant defense system.