Preparation of the L-Asparaginase-Based Biosensor With Polyimide Membrane Electrode for Monitoring L-Asparagine Levels in Leukemia

ERDOĞAN A., KÖYTEPE S., ATEŞ B., YILMAZ İ., SEÇKİN T.

INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS, cilt.63, sa.17, ss.909-917, 2014 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 63 Sayı: 17
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1080/00914037.2014.886228
  • Dergi Adı: INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.909-917
  • Anahtar Kelimeler: Biosensor, immobilization, L-asparaginase, L-asparagine, polyimide membrane, GLUCOSE-OXIDASE, IMMOBILIZATION, COPOLYMERS, POLYMERS, MATRIX, BLOOD
  • İnönü Üniversitesi Adresli: Evet

Özet

Polyimide based amperometric biosensor was developed for the in vitro determination of L-asparagine in serum samples for monitoring L-asparagine levels in leukemia. First, polyimide from pyromellitic dianhydride and 4,4-oxydianiline (ODA) was prepared. This prepared polyimide was used as an immobilization membrane of L-asparaginase (L-ASNase) for L-ASNase-polyimide-coated electrodes. From the amperometric results for the polyimide electrode, it is concluded that polyimide membrane containing L-asparaginase can be used as a membrane for asparagine detection in the presence of large concentration of interferences, because of its strong adherence to the electrode surface, easy preparation, chemical stability, selectivity, and very high statistical confidence (R=0.9988).