Detection of 2019-nCoVN2 of SARS CoV2 by Lateral Flow Assay


Çam Derin D., Gültekin E., İçen Taşkın I., Otlu B.

4. EBAT, Antalya, Türkiye, 3 - 06 Kasım 2022, ss.15-16

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Antalya
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.15-16
  • İnönü Üniversitesi Adresli: Evet

Özet

The molecular detection of SARS CoV_2 is the most reliable method in the world and it is performed by real time reverse transcriptase polymerase chain reaction (rRT-PCR ) for the conserved regions of viral genome, which was announced by the World Health Organisation. One of these region is 2019-nCoV_N2 and this sequence is still used for molecular detection as different base lengths depending on the kit suppliers. Since the rRT-PCR method is expensive and needs well trained personnel, alternative molecular detection tools are needed. Lateral flow assay is a naked eye analysis test used for nucleic acid detection as a molecular assay and commonly developed by gold nanoparticles.

 

In this study, lateral flow assay platform was designed and prepared for the molecular recognition of 2019-nCoV_N2 region by using gold nanoparticles as label. Assay was prepared according to the principle of hybridization of synthetic oligonucleotides on the membrane. Strip tests are prepared by synthesized colloidal gold nanoparticle/single stranded oligonucleotide conjugate and commercially available nitrocellulose membrane.

 

Results showed that both the synthetic target sequence and real sample sequence of 2019-nCoV_N2 obtained from the patients were detected by naked eye in a 5 minute with the prepared strip assay. Therefore, the usage of lateral flow assay for the molecular detection of SARS CoV_2 as an alternative and cheap method to rRT-PCR was demonstrated, successfully. It was also hypothesized that this sandwich model can be prepared and used for the detection of polymerase chain reaction products of virus and could be adopted to mutated regions of viral genome as a further goals.