In this study, our hypothesis was that workplace noise can alter brain element levels like immobilization and light-dark cycle shift, and we aimed to investigate the effects of workplace noise on element levels of brain areas as well as changes in blood of chronically noise-exposed rats. Twenty-four rats were randomly divided into three groups. The first group was the control. The second group (noise exposed) was subjected to daily 8 h 85 dB workplace noise for 15 days, and the third group (noise exposed plus normal condition) was also subjected to the same noise exposure and then returned to normal condition for 15 days. In noise-exposed group, Na, K, Ca, Mg , Fe, Cu, and Zn levels in plasma significantly increased when compared with controls (p = 0.03 for Na; p = 0.005 for K and Mg; p = 0.002 for Ca and Fe; p = 0.01 for Cu and Zn). In this group, Fe level of temporal lobe significantly increased (p = 0.021) while Mg level significantly increased in frontal (p = 0.021) and temporal (p = 0.001) lobes when compared with controls. In the noise-exposed plus normal condition group, plasma Na, K, Fe, Cu, and Zn levels were greatly similar to controls. But, plasma Ca and Mg levels significantly decreased when compared with controls (p = 0.002 and p = 0.007) and noise-exposed group (p = 0.002 and p = 0.001). Fe level of temporal lobe significantly increased when compared with controls (p = 0.001) and noise exposed group (p = 0.001). These alterations may be attributed to impaired intake of water and micronutrients or their excretions, emerging by acoustic stress.