Design of highly selective, and sensitive screen-printed electrochemical sensor for detection of uric acid with uricase immobilized polycaprolactone/polyethylene imine electrospun nanofiber

Muhammad F., Dik G., Kolak S., Gedik K. K., Bakar B., ULU A., ...Daha Fazla

Electrochimica Acta, cilt.439, 2023 (SCI-Expanded, Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 439
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1016/j.electacta.2022.141675
  • Dergi Adı: Electrochimica Acta
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, INSPEC, Metadex, Civil Engineering Abstracts
  • Anahtar Kelimeler: Biosensor, Differential pulse voltammetry, Electrospun nanofiber, Enzyme immobilization, Quantum dot screen printed electrode, Uric acid, Uricase
  • İnönü Üniversitesi Adresli: Evet

Özet

Uric acid (UA) plays a significant role in nerve center, human metabolism, and kidney system. Therefore, it is necessary to establish a simple, rapid, and sensitive detection method for UA. In recent years, researchers have been highly attracted by nanomaterials with satisfactory functions in electrochemical applications. In this study, polycaprolactone (PCL)/polyethylene imine (PEI) nanofiber membranes were prepared and used for the immobilization of uricase (UOx). The prepared membranes were characterized in terms of structure, composition, and morphology. Afterward, the quantum dot screen printed electrode (QD SPCE) was modified with PCL/PEI nanofiber membranes with and without methylene blue (MB) as an electron mediator. The electrochemical performance of the developed sensors was investigated by cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS). Under the optimal conditions, the modified sensors provided a broad linear range (5.0–52.0 µM) for the electrochemical detection of UA. Limit of detection (LoD) values were determined as 3.96 µM and 1.85 µM for the PCL/PEI/UOx/QD SPCE and PCL/PEI/UOx/MB/QD SPCE, respectively. The four-week stability results showed the change in current for the PCL/PEI/UOx/QD SPCE and PCL/PEI/UOx/MB/QD SPCE to be 92% and 87%, respectively. Additionally, in the mixed interference test remaining current ratios for UA were 95% and 82% for the PCL/PEI/UOx/QD SPCE and PCL/PEI/UOx/MB/QD SPCE, respectively. Most importantly, the effectiveness of the electrodes was also verified in real sample detection with satisfactory recovery (∼98-112%). Overall, the results showed that the PCL/PEI/UOx/QD SPCE and PCL/PEI/UOx/MB/QD SPCE have the advantages of simplicity, high sensitivity, and good selectivity for UA determination.