The main aim of this study is to define the personality traits of strategic entrepreneurs'. Due to this aim there have been established 1 main and 5 sub-hypothesis in this study. The sample of strategic entrepreneurs' is created from the biggest 500 firms' list in Turkey. The companies are choosen from Kocaeli, Istanbul, Izmir, Ankara and Bursa. The survey is applied on 105 strategic entrepreneurs. After collecting data it is examined in SPSS so the main hypethesis is verified partially. And the sub-hypothesises are verified partially too. In this study, Neo - FFI personality inventory is used to measure personality for a responsive result.