Simultaneous Determination of Water and Fat-Soluble Vitamins in Pekmez Samples by High Performance Liquid Chromatography Coupled with Diode Array Detection


ASIAN JOURNAL OF CHEMISTRY, cilt.22, ss.4083-4091, 2010 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 22 Konu: 5
  • Basım Tarihi: 2010
  • Sayfa Sayıları: ss.4083-4091


In the present work, a RP-HPLC procedure has been applied for the determination of water-soluble vitamins (riboflavin. nicotinamide. pantothenic acid. pyridoxine hydrochloride. pyridoxal. folic acid, ascorbic acid) and tat-soluble vitamins (trans-retinol. alpha-tocopherol) in pekmez samples. a tradional Turkish concentrate fruit food The sample treatment proposed consists of a solid-phase extraction with Sep-Pak C(18) cartridges that allow the seperation of fat-soluble vitamins, which were retained on the sorbent, from water-soluble vitamins. After isolation of the two fractions, the water-soluble vitamins were analyzed by HPLC on an ACE 5 C(18) (250 x 4.6 mm, 5 mu m) analytical column using 0.1 mol L(-1) KH(2)PO(4) (pH 7 0)-CH(3)OH as mobile phase The chromatographic analysis of the fat-soluble vitamins was earned out after their sequential elution with CH(3)OH-CCl(4) from C(18) sorbent. on the above column. Afterwards. the fat-soluble vitamins were analyzed on an ACE 5 C(18) analytical with isocratic mobile phase consisting of CH(3)OH-CH(3)CN at a flow-rate of 2 mL min(-1). Identification of compounds was achieved by comparing their retention times and UV spectra with those of standards stored in a data bank The accuracy of the method was tested obtaining an average recovery ranging between 91 and 110%.