Synthesis and characterization of biodegradable pHEMA-starch composites for immobilization of L-asparaginase

ULU A., KÖYTEPE S., ATEŞ B.

POLYMER BULLETIN, cilt.73, sa.7, ss.1891-1907, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 73 Sayı: 7
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1007/s00289-015-1583-1
  • Dergi Adı: POLYMER BULLETIN
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1891-1907
  • Anahtar Kelimeler: L-asparaginase, pHEMA, pHEMA-starch composites, Immobilization, ACUTE LYMPHOBLASTIC-LEUKEMIA, 2-HYDROXYETHYL METHACRYLATE, NANOCOMPOSITE FILM, IN-VITRO, PROTEIN, POLYMERIZATION, STABILIZATION, NANOPARTICLES, BIOSENSOR, HYDROGELS
  • İnönü Üniversitesi Adresli: Evet

Özet

In this paper, poly (2-hydroxyethyl methacrylate) (pHEMA) and poly (2-hydroxyethyl methacrylate)-starch (pHEMA-S) composites were prepared from 2-hydroxyethyl methacrylate and different ratio starch. The pHEMA-S showed high thermal stability and biodegradability. The biodegradability of pHEMA-S composites was in the range of 14-22 % for 4 weeks depending on the starch ratio of pHEMA-S. Then, L-ASNase was immobilized onto pHEMA-S composites. The immobilized L-ASNase was investigated with optimum temperature, pH and kinetic parameters. Immobilization improved the pH stability and thermal stability of the enzyme. The K-m values were 1.58 and 0.56 mM for free and immobilized enzyme, respectively.