Biochemical and developmental effects of thyroid and anti-thyroid drugs on different early life stages of Xenopus laevis

BORAN F., GÜNGÖRDÜ A.

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, cilt.87, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 87
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.etap.2021.103738
  • Dergi Adı: ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, Environment Index, Greenfile, MEDLINE, Pollution Abstracts, Veterinary Science Database
  • Anahtar Kelimeler: Levothyroxine, Propylthiouracil, Xenopus laevis, Endocrine disruptor chemicals, Biomarkers, ENVIRONMENTAL RISK, PROPYLTHIOURACIL, METHIMAZOLE, EXPRESSION, EXPOSURE, LIVER, ASSAY, ACETYLCHOLINESTERASE, HYPERTHYROIDISM, HYPOTHYROIDISM
  • İnönü Üniversitesi Adresli: Evet

Özet

The effects of two drugs containing the synthetic thyroid hormone levothyroxine (LEV) and an anti-thyroid drug containing propylthiouracil (PTU) on the three early life stages of Xenopus laevis were evaluated with the Frog Embryo Teratogenesis Assay-Xenopus, Tadpole Toxicity Test, and Amphibian Metamorphosis Assay using biochemical and morphological markers. Tested drugs caused more effective growth retardation in stage 8 embryos than stage 46 tadpoles. Significant inhibition of biomarker enzymes has been identified in stage 46 tadpoles for both drugs. AMA test results showed that LEV-I caused progression in the developmental stage and an increase in thyroxine level in 7 days exposure and growth retardation in 21 days exposure in stage 51 tadpoles. On the other hand, increases in lactate dehydrogenase activity for both drugs in the AMA test may be due to impacted energy metabolism during sub-chronic exposure. These results also show that the sensitivity and responses of Xenopus laevis at different early developmental stages may be different when exposed to drugs.