In this study, the protective role of organic selenium against salt stress on Hordeum vulgare L. and Triticum aestivum L. were determined. Seeds, treated with 0.004 M organic selenium in the pre-application, were germinated in environments exposed to 50, 100 and 200 mM salt stress (NaCl), in comparison to seeds without selenium treatment. The effects of 0.004 M organic selenium on peroxidase, ascorbate peroxidase, and lipid peroxidation were investigated in both plants. An evaluation of their germination and enzyme activities showed that organic selenium resulted in salient improvement when compared to the plants directly exposed to salt stress. The present study suggests that selenium can play a protective role during salt stress by enhancing the antioxidant defense system.