ASSESSMENT OF PHYTOTOXIC AND GENOTOXIC EFFECTS OF ANATASE TiO2 NANOPARTICLES ON MAIZE CULTIVAR BY USING RAPD ANALYSIS


MUTLU F., YÜREKLİ F., MUTLU B., EMRE F. B., OKUŞLUK F., ÖZGÜL O.

FRESENIUS ENVIRONMENTAL BULLETIN, cilt.27, sa.1, ss.436-445, 2018 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 27 Sayı: 1
  • Basım Tarihi: 2018
  • Dergi Adı: FRESENIUS ENVIRONMENTAL BULLETIN
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.436-445
  • Anahtar Kelimeler: RAPD, genotoxic effects, Zea mays, nano-TiO2, genomic template stability, germination seedling, growth parameters, TITANIUM-DIOXIDE NANOPARTICLES, AMPLIFIED POLYMORPHIC DNA, ZEA-MAYS L., OXIDE NANOPARTICLES, SEED-GERMINATION, ZINC-OXIDE, NANO-TIO2, PLANT, TOXICITY, SOIL
  • İnönü Üniversitesi Adresli: Evet

Özet

In this study, early phytotoxic and genotoxic effects of chemically synthesized nano-TiO2 (anatase) were investigated in seedlings of maize (Zea mays cv. Hido). Control (Hoagland) and NaCl (300 mM) groups were administered with nano-TiO2 (0.1%, 0.2% and 0.3%). Seed germination and seedling growth parameters of maize cultivar were markedly inhibited by salt stress. Nano-TiO2, without depending on concentration, was found to be ineffective on germination percentage in all administrations. On the contrary, nano-TiO2 caused significant increases in root-stem length and fresh-dry weights especially in NaCl+nano-TiO2 administrations. In salt administered samples, 0.3% nano-TiO2 increased root length 1.4 fold, stem length 4.8 fold, and fresh weight 1.2 fold. Genotoxic properties of nano-TiO2 in seedlings of maize were evaluated by using randomly amplified polymorphic DNA (RAPD-PCR). In RAPD analysis, 20 RAPD primers were found to produce unique polymorphic band profiles at different concentrations of nano-TiO2 maize seedlings. Genomic template stability (GTS), a qualitative measurement of changes in RAPD patterns of genomic DNA, decreased depending on increasing nano-TiO2, NaCl, and NaCl+nano-TiO2 concentrations. Therefore, it could be concluded that nano-TiO2 of appropriate dose can be used to ameliorate negative effects of salt stress by increasing growth potential of maize.