Effects of molsidomine on retinal ischemia/reperfusion injury in rabbits


Polat N., Ozer M. A. , Parlakpinar H. , Vardi N., Aksungur Z., Ozhan O., ...Daha Fazla

BIOTECHNIC & HISTOCHEMISTRY, cilt.93, ss.188-197, 2018 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 93 Konu: 3
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1080/10520295.2017.1406616
  • Dergi Adı: BIOTECHNIC & HISTOCHEMISTRY
  • Sayfa Sayıları: ss.188-197

Özet

We investigated the effect of molsidomine (MOL) on ischemia/reperfusion (I/R) injury. Rabbits were assigned to four groups: group 1, sham; group 2, I/R; group 3, MOL treatment for 4days after I/R; group 4, MOL treatment for 1 day before I/R and 3days after I/R. Retinal I/R was produced by elevating the intraocular pressure to 150mm Hg for 60min. Seven days after I/R, the eyes were enucleated. Retinal changes were examined using histochemistry. The levels of malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) also were measured. We found a significant increase in the thickness of the outer nuclear layer of group 3 compared to the other groups. In groups 3 and 4, caspase-3 stained cells in the ganglion cell layer were decreased compared to group 2. We found a significant increase in caspase-3 stained cells in the inner nuclear layer (INL) of group 2 compared to the other groups. We found a significant increase in caspase-3 stained cells in group 3 compared to group 4 in the INL. The MDA level in group 2 was significantly higher than group 1 and MOL significantly decreased MDA levels in groups 3 and 4. We found that MOL protected the retina from I/R injury by enhancing antioxidative effects and inhibiting apoptosis of retinal cells.