The lactic acid bacteria are known to be probiotic and their important role has been known in making the biotechnological products and improving human health. Effective probiotic should be viable, safe, bile and gastric juices tolerant, able to survive throughout the human gastrointestinal tract and to colonize a specific human tract. We aimed to investigate and compare the probiotic potential of the Lactobacillus helveticus ATCC 15009 (L. helveticus), Lactobacillus plantarum ATCC 14917 (L. plantarum), Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 (L. bulgaricus), Lactococcus lactis IL1403 (L. lactis IL1403) and Lactococcus lactis IL1403 bearing pSIP plasmid (L. lactis IL403 pSIP) in different aspects that include their ability to tolerate acidic conditions, gastric acid and intestinal juice. We also evaluated their hydrophobicity and antibiotic sensitivity.