The solvent effect of ethanol and n-hexane on the hydrolysis of triolein and tributyrin by Candida cylindracea lipase


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Koç A., Özer D., Ekiz H. İ.

NEW BIOTECHNOLOGY, vol.25, pp.166, 2009 (SCI-Expanded)

  • Publication Type: Article / Letter
  • Volume: 25
  • Publication Date: 2009
  • Journal Name: NEW BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.166
  • Inonu University Affiliated: Yes

Abstract

In this study, triolein and tributyrin were used as substrates and ethanol and n-hexane were used as solvents in enzyme catalytic hydrolysis of triglycerides. The initial reaction rate of triolein hydrolysis, increased up to 0.00516 mol L−1 of triolein concentration then it decreased. The maximum initial reaction rate of triolein hydrolysis was found 17.850 and 9.425 without ethanol and presence ethanol media 2% (v/v), respectively. Thus ethanol was effected mixed type inhibitor of initial reaction rate on triolein hydrolysis. The hydrolysis reaction rate of tributyrin increased up to 0.0513 mol L−1of tributyrin concentration then it decreased slightly. The maximum initial reaction rate of tributyrin hydrolysis found 180.52 and 103.09 without n-hexane and at presence n-hexane media 2% (v/v), respectively. Thus n-hexane effected uncompetitive inhibitor of initial reaction rate on tributyrin hydrolysis.