Research Information System
NEW BIOTECHNOLOGY, vol.25, pp.166, 2009 (SCI-Expanded)
In this study, triolein and tributyrin were used as substrates and ethanol and n-hexane were used as solvents in enzyme catalytic hydrolysis of triglycerides. The initial reaction rate of triolein hydrolysis, increased up to 0.00516 mol L−1 of triolein concentration then it decreased. The maximum initial reaction rate of triolein hydrolysis was found 17.850 and 9.425 without ethanol and presence ethanol media 2% (v/v), respectively. Thus ethanol was effected mixed type inhibitor of initial reaction rate on triolein hydrolysis. The hydrolysis reaction rate of tributyrin increased up to 0.0513 mol L−1of tributyrin concentration then it decreased slightly. The maximum initial reaction rate of tributyrin hydrolysis found 180.52 and 103.09 without n-hexane and at presence n-hexane media 2% (v/v), respectively. Thus n-hexane effected uncompetitive inhibitor of initial reaction rate on tributyrin hydrolysis.