Comparison of the selenium level with GSH-Px activity in the liver of mice treated with 7,12 DMBA

Batcioglu K., Ozturk C., Karagozler A., Karatas F.

CELL BIOCHEMISTRY AND FUNCTION, cilt.20, ss.115-118, 2002 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 20 Konu: 2
  • Basım Tarihi: 2002
  • Doi Numarası: 10.1002/cbf.958
  • Sayfa Sayıları: ss.115-118


In this study, the relationship between selenium (Se) and glutathione peroxidase (GSHPx) levels was investigated in 7,12-dimethylbenz(a)anthracene (7,12-DMBA)-treated mouse liver. The potential mammary carcinogen 7,12-DMBA, was injected intraperitoneally (20 mg kg(-1) day(-1)) into 10-12 month old female mice. After 21 days of application the mice were sacrificed and GSHPx and Se levels of Liver homogenates were measured. Se and GSHPx levels in 7,12-DMBA-treated mice were significantly lower than those of controls (p<0.05). The control group exhibited 0.9 +/- 0.066 U mg(-1) protein and 0.86 +/- 0.058 p.p.m. levels of GSHPx and Se respectively. The 7,12-DMBA-treated group had significantly (p<0.05) decreased GSHPx and Se levels (0.42+/-0.062 U mg(-1) protein and 0.69+/-0.034 p.p.m. respectively). The results show a direct relationship between Se and GSHPx activity and a negative correlation between antioxidant capacity and existence of a carcinogen in metabolism. Copyright (C) 2001 John Wiley Sons, Ltd.