Synthesis, Characterization, DNA Binding and Anticancer Activities of The Imidazolidine-Functionalized (NHC)Ru(II) Complexes

Gürses C., Aktaş A., Balcıoğlu S., Fadhilah A., Gök Y., Ateş B.

JOURNAL OF MOLECULAR STRUCTURE, cilt.131350, sa.1247, ss.1-9, 2022 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 131350 Sayı: 1247
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.molstruc.2021.131350
  • Dergi Adı: JOURNAL OF MOLECULAR STRUCTURE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Chimica, Compendex, INSPEC
  • Sayfa Sayıları: ss.1-9
  • İnönü Üniversitesi Adresli: Evet

Özet

Here, we present a study on the synthesis and biological properties of the ruthenium complexes hav- ing naphthyl substituted NHC ligands, which are of great importance for drug development studies. For this purpose, in our study, imidazolidine-functionalized Ru(II)NHC complexes containing naphthyl sub- stitute group were synthesized and in vitro anticancer activity as well as DNA binding properties us- ing agarose gel electrophoresis and ethidium bromide fluorescence quenching assay methods were de- termined in order to understand the biological activities. The Ru(II)NHC complexes were prepared from naphthyl-substituted Ag(I)NHC complexes and [RuCl 2 ( p -cymene)] 2 via the transmetallation method. These new complexes were characterized by using 1 H NMR, 13 C NMR, FTIR spectroscopic method and elemen- tal analysis techniques. Consequently, complex 1d showed the highest anticancer activity in terms of IC 30 and IC 50 values against HepG2 cell line as 23.9 and 32.84 μM, respectively. Moreover, depending on the data obtained from agarose gel electrophoresis and fluorescence spectrophotometry techniques, 1b had the highest DNA binding property, which means that 1b behaved binding DNA in the cell as an intercala- tive manner rather than disrupting the integrity of cell membrane like 1d.