In this study, 128 test strains included 72 representatives of major membered Streptomyces clusters circumscribed by Williams et al. (1983) and 56 representative Streptomyces group A, B and C strains were circumscirbed using fluorogenic enzyme data. The representatives of Streptomyces groups A, B and C formed discrete homogeneous clusters that were sharply separated from the marker strains representing the 72 major Streptomyces clusters. There is, however, little evidence that the marker strains grouping into the numerically circumscribed clusters reported by Williams et al. (1983). This study show that rapid enzyme tests could used to identify unknown strains isolated from any environment.