In this study, an electrochemical biosensor was developed by using a sol-gel coating solution. The modified platinum electrode used in the study was constructed by immobilization of glucose oxidase under a layer of sol-gel film. The sol-gel coating solution was prepared by using GLYMO, TEOS, and MTEOS. Electrochemical measurements were carried out amperometrically by determining hydrogen peroxide produced by the enzymatic reaction between glucose and glucose oxidase. The amperometric responses of the resulting enzymatic electrode to glucose were rapid. It was observed that the amperometric response of the enzymatic electrode was linear for glucose concentrations in the range from 2 to 18 mM with 50 s response time. LOD and LOQ for the enzymatic electrode were calculated to be 0.055 mM and 0.184 mM, respectively. It was determined that the developed biosensor had an acceptable reproducibility. The selectivity of the biosensor was determined in the presence of some interfering substances, such as lactose, sucrose, urea, uric acid, oxalic acid, and ascorbic acid. The stability of the biosensor was investigated, and it was found that the sensor response decreased by 59% of its initial response over a period of 30 days of storage in dry conditions at 4 degrees C.