Comparing DPPP fluorescence and UV based methods to assess oxidation degree of krill oil-in-water emulsions

ULUATA S., DURMAZ G., McClements D. J., Decker E. A.

FOOD CHEMISTRY, cilt.339, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 339
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.foodchem.2020.127898
  • Dergi Adı: FOOD CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, EMBASE, Food Science & Technology Abstracts, MEDLINE, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Anahtar Kelimeler: Lipid oxidation, Krill oil, DPPP, Fluorescence methods, Emulsion, NONENZYMATIC BROWNING REACTIONS, LIPID OXIDATION, FISH-OIL, STABILITY, NANOEMULSIONS, ANTIOXIDANTS, AUTOXIDATION, PEROXIDATION, CHEMISTRY, PRODUCTS
  • İnönü Üniversitesi Adresli: Evet

Özet

In this study, lipid oxidation evaluation methods were compared for a krill-oil-in-water emulsion system. With this aim, thiocyanate and DPPP (diphenyl-1-pyrenylphosphine) fluorescence methods were comparatively examined to determine primary oxidation products. 2-thiobarbituric acid reactive substances (TBARS), hexanal and propanal formation were also monitored as secondary oxidations products. All oxidation experiments were performed via both auto-oxidation at 45 degrees C and light-riboflavin induced photooxidation at 37 degrees C. The results have shown that thiocyanate method was not suitable to measure lipid hydroperoxides by the both in auto- and photo-oxidation systems. On the other hand, fluorescence intensity of samples containing the DPPP probe increased during incubation period which indicates the formation of lipid hydroperoxides could be detected via this method. TBARS, hexanal and propanal concentrations also increased during storage period and the formation kinetics of secondary oxidation products was confirmed that the DPPP fluorescence method was accurate and reliable at different environmental conditions.