Akademik Veri Yönetim Sistemi
PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES, cilt.37, sa.1, ss.25-32, 2024 (SCI-Expanded)
In the present study, antioxidant activity and inhibition of acetylcholinesterase (AChE) and paraoxonase
(hPON 1) of Alchemilla lithophila extracts were evaluated for the first time. Besides, there is no research on the contents
of phenolic compounds except for fatty acids. In this context, phenolic compounds of A. lithophila were investigated by
liquid chromatography/ mass spectrometry (LC-MS/MS). The methanol extract of the A. lithophila exhibited significant
inhibition on the AChE (IC50 value for methanol extract 0.162 ± 0.25 mg /mL, R2
:0.992). Besides, antioxidant activities
of the A. lithophila extracts were examined using by the methods ABTS•+and DPPH•
free radical scavenging potentials,
FRAP and CUPRAC metal-reducing activities. ABTS•+ and DPPH•
scavenging activities were found for methanol
extract at 70.67% and water extract at 75.38%, respectively. Also, FRAP and CUPRAC metal-reducing were determined
for water extract 0.796 and hexane extract 1.570 as absorbance. According to LC-MS/MS analyses, the amounts of
ellagic acid, catechin hydrate, gallic acid, fumaric acid, luteolin, quercetin, kaempferol, acetohydroxamic acid, caffeic
acid, syringic acid, hydroxybenzoic acid and salicylic acid were determined by LC-MS/MS, respectively. As a
consequence, this study will be a useful resource for determining bioactivity and phenolic compound profile for natural
medicine research.