Design of starch functionalized biodegradable P(MAA-co-MMA) as carrier matrix for L-asparaginase immobilization

ULU A., KÖYTEPE S., ATEŞ B.

CARBOHYDRATE POLYMERS, cilt.153, ss.559-572, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 153
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1016/j.carbpol.2016.08.019
  • Dergi Adı: CARBOHYDRATE POLYMERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.559-572
  • Anahtar Kelimeler: L-Asparaginase, Enzyme immobilization, P(MAA-co-MMA), Starch, Biocomposite, POLYMETHACRYLIC ACID, DRUG-DELIVERY, METHYL-METHACRYLATE, CHITOSAN, RELEASE, LEUKEMIA, SYSTEMS, NANOPARTICLES, DEGRADATION, COMPOSITE
  • İnönü Üniversitesi Adresli: Evet

Özet

We prepared biodegradable P(MAA-co-MMA)-starch composite as carrier matrix for the immobilization of L-asparaginase (L-ASNase), an important chemotherapeutic agent in acute lymphoblastic leukemia. Chemical characteristics and thermal stability of the prepared composites were determined by FT-IR, TGA, DTA and, DSC, respectively. Also, biodegradability measurements of P(MAA-co-MMA)-starch composites were carried out to examine the effects of degradation of the starch. Then, L-ASNase was immobilized on the P(MAA-co-MMA)-starch composites. The surface morphology of the composite before and after immobilization was characterized by SEM, EDX, and AFM. The properties of the immobilized L-ASNase were investigated and compared with the free enzyme. The immobilized L-ASNase had better showed thermal and pH stability, and remained stable after 30 days of storage at 25 degrees C. Thus, based on the findings of the present work, the P(MAA-co-MMA)-starch composite can be exploited as the biocompatible matrix used for L-ASNase immobilization for medical applications due to biocompatibility and biodegradability. (C) 2016 Elsevier Ltd. All rights reserved.