Akademik Veri Yönetim Sistemi
Biochemical Engineering Journal, cilt.233, 2026 (SCI-Expanded, Scopus)
Adipose tissue is one of the loose, specialized connective tissues found in mammals and it plays a key role in energy storage, immune defense, endocrine function, thermogenesis, and thermal insulation. In that sense, modeling the adipose tissue in vitro plays a huge role in further enlightening mechanisms behind the diseases and adipogenesis. We successfully generated mature white adipose tissue (WAT) spheroids from an induced pluripotent stem cell (iPSC)-derived neuromesodermal progenitors (NMPs) for the first time in the literature to our knowledge. After characterizing the iPSCs and iPSC-derived NMPs, iPSC-derived mature WAT spheroids were generated using the differentiation protocol developed by our group. Detailed characterization of WAT spheroids showed that spheroids were expressing high levels of adipogenic genes alongside having a high capacity for lipid storage. Secretome characterization of WAT spheroids showed that their secretome contained extracellular vesicle-like structures and had high triglyceride content. To further assess the potential effects of the (conditioned medium) CM of WAT spheroids on the metabolism and adipogenesis process, we treated β-TC-6 and ASC52-telo with the spheroids’ CM. The data showed that the CM supplementation to β-TC-6 cells increased insulin secretion, while no significant alternations were observed in adipogenesis of ASC52-telo cells. Overall, the findings of this study highlight the generation of iPSC-derived WAT spheroids and the detailed characterization of both the spheroids and their secretome.