The in vitro toxicity analysis of titanium dioxide (TiO2) nanoparticles on kinematics and biochemical quality of rainbow trout sperm cells


Ozgur M. E. , Balcioglu S., Ulu A., Ozcan I., Okumus F., KÖYTEPE S., ...More

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, vol.62, pp.11-19, 2018 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 62
  • Publication Date: 2018
  • Doi Number: 10.1016/j.etap.2018.06.002
  • Journal Name: ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.11-19
  • Keywords: TiO(2)nanoparticles, Rainbow trout, Sperm cell kinematics, Oxidative stress markers, OXIDATIVE STRESS, MOTILITY PARAMETERS, ANALYSIS CASA, ZEBRAFISH, FISH, EXPOSURE, CYTOTOXICITY, CADMIUM, ANATASE, DAMAGE
  • Inonu University Affiliated: Yes

Abstract

In recent years, titanium dioxide (TiO2) nanoparticles (NPs) as metal oxide nanoparticles are widely used in industry, agriculture, personal care products, cosmetics, sun protection and toothpaste, electronics, foodstuffs and food packaging. This use of nano-TiO2 has been associated with environmental toxicity concerns. Therefore, the aim of this study was to evaluate the in vitro effect of different doses of TiO2 NPs (similar to 30-40 nm) (0.01, 0.1, 0.5, 1, 10 and 50 mg/L) at 4oC for 3 h on the sperm cell kinematics as velocities of Rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) sperm cells. Furthermore, oxidative stress markers (total glutathione (TGSH) and superoxide dismutase (SOD) were assessed in sperm cells after exposure to TiO2 NPs. According to the obtained results, there were statistically significant (P < 0.05) decreasing in the velocities of sperm cells after 10 mg/L TiO2 NPs and an increase the activity of SOD (P < 0.05) and TGSH levels were determined.