Evaluation of the biochemical effects of an acetamiprid-based insecticide on a non-target species,Gambusia holbrooki

DEMİRCİ Ö., GÜNGÖRDÜ A.

WATER AND ENVIRONMENT JOURNAL, cilt.34, ss.481-489, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 34
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1111/wej.12549
  • Dergi Adı: WATER AND ENVIRONMENT JOURNAL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.481-489
  • Anahtar Kelimeler: biomarker, integrated biomarker response, pesticides pollution, GLUTATHIONE-S-TRANSFERASE, LACTATE-DEHYDROGENASE, IN-VITRO, ACETYLCHOLINESTERASE ACHE, CARBOXYLESTERASE ACTIVITY, LIPID-PEROXIDATION, OXIDATIVE STRESS, DAPHNIA-MAGNA, CATALASE CAT, TOXICITY
  • İnönü Üniversitesi Adresli: Evet

Özet

The toxic effects of an acetamiprid-based insecticide (ABI) onGambusia holbrookiwere evaluated after 24 and 96-h exposure periods. The 24 and 96-h median lethal concentration (LC50) values of ABI were determined as 75.9 and 42.2 mg/L active ingredient (AI)/L, respectively. In addition, the activity of five biochemical marker enzymes, including glutathione S-transferase (GST), glutathione reductase (GR), carboxylesterase (CaE), lactate dehydrogenase (LDH) and aspartate aminotransferase (AST) was measured after 24 and 96-h exposure to three different concentrations of the ABI to evaluate its sublethal effects. The acetamiprid concentrations in the exposure media were measured using liquid chromatography-tandem mass spectrometry. Our results showed that GST and LDH activities were increased and there were concentration-dependent changes in the integrated biomarker response (IBR) indexes after 24-h ABI exposure. However, the examined biomarkers were not useful for examining the effects of the ABI exposure for 96-h exposure periods, even at the highest concentration.