This study aims to investigate the effects of electromagnetic radiation (EMR) delivered by mobile phones on oxidative stress, histological damage and sperm characteristic in rats. For this purpose, 18 male rats (9 in the study group and 9 in the control group) were used. The rats in experimental group were exposed to EMR from an active (1.9-2.2 GHz) mobile phone (MP) for 20 min per day for 20 days. The rats in control group were exposed to an MP without a battery for the same period. The results showed that there was no significant differences in oxidative stress parameters (TBARS, GSH, SOD, CAT, GSH-Px), testis histology and reproductive organ weight (testis, epididymis, prostate weight) between the control and the study groups. On the other hand, it was observed that the use of MPs may have negative effects on the sperm characteristics (especially sperm motility) in rats. In conclusion, it was suggested that the exposure of EMR caused a decrease of fertility capacity due to their effects on sperm characteristics.