Investigation of therapeutic effects in the wound healing of chitosan/pGM-CSF complexes

ŞALVA E., ALAN S., Karakoyun B., Cakalagaoglu F., ÖZBAŞ S., Akbuga J.

BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES, cilt.58, 2022 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 58
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1590/s2175-97902022e19668
  • Dergi Adı: BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, EMBASE, Veterinary Science Database, Directory of Open Access Journals
  • Anahtar Kelimeler: Wound healing, Chitosan, pGM-CSF, Complexes, COLONY-STIMULATING FACTOR, MOLECULAR-WEIGHT, GROWTH-FACTORS, GENE, NANOPARTICLES, DELIVERY, PROLIFERATION, POLYPLEXES, EFFICIENCY, CYTOKINES
  • İnönü Üniversitesi Adresli: Evet

Özet

Granulocyte macrophage colony-stimulating factor (GM-CSF) has been shown to promote the growth, proliferation, and migration of endothelial and keratinocyte cells. Chitosan has been widely used as a biopolymer in wound-healing studies. The aim of this study was to investigate the in vitro proliferative effects of chitosan/pGM-CSF complexes as well as the therapeutic role of the complexes in an in vivo rat wound model. The effect of complexes on cell proliferation and migration was examined. Wounds were made in Wistar-albino rats, and examined histopathologically. The cell proliferation and migration were increased weight ratio- and time-dependently in HaCaT and NIH-3T3 cell lines. Wound healing was significantly accelerated in rats treated with the complexes. These results showed that the delivery of pGM-CSF using chitosan complexes could play an accelerating role in the cell proliferation, migration, and wound-healing process.