Thioredoxin is required for deoxyribonucleotide pool maintenance during S phase


Koc A. , Mathews C. K. , Wheeler L. J. , Gross M. K. , Merrill G. F.

JOURNAL OF BIOLOGICAL CHEMISTRY, cilt.281, ss.15058-15063, 2006 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 281 Konu: 22
  • Basım Tarihi: 2006
  • Doi Numarası: 10.1074/jbc.m601968200
  • Dergi Adı: JOURNAL OF BIOLOGICAL CHEMISTRY
  • Sayfa Sayıları: ss.15058-15063

Özet

Thioredoxin was initially identified by its ability to serve as an electron donor for ribonucleotide reductase in vitro. Whether it serves a similar function in vivo is unclear. In Saccharomyces cerevisiae, it was previously shown that Delta trx1 Delta trx2 mutants lacking the two genes for cytosolic thioredoxin have a slower growth rate because of a longer S phase, but the basis for S phase elongation was not identified. The hypothesis that S phase protraction was due to inefficient dNTP synthesis was investigated by measuring dNTP levels in asynchronous and synchronized wild-type and Delta trx1 Delta trx2 yeast. In contrast to wild-type cells, Delta trx1 Delta trx2 cells were unable to accumulate or maintain high levels of dNTPs when alpha-factor- or cdc15-arrested cells were allowed to reenter the cell cycle. At 80 min after release, when the fraction of cells in S phase was maximal, the dNTP pools in Delta trx1 Delta trx2 cells were 60% that of wild-type cells. The data suggest that, in the absence of thioredoxin, cells cannot support the high rate of dNTP synthesis required for efficient DNA synthesis during S phase. The results constitute in vivo evidence for thioredoxin being a physiologically relevant electron donor for ribonucleotide reductase during DNA precursor synthesis.