Because of their many advantages, adhesively bonded joints are intensively used in many engineering fields. So, the mechanical research of the adhesively bonded joints is very important to use these joints safely. There are many studies performed by researchers to investigate the mechanical properties of the adhesive joints. There has been a considerable interest in nanoparticles added to structural adhesives recently because nanoparticles improve the mechanical properties of adhesives and joints. In this paper, different nanoparticles reinforced by epoxy adhesive, and neat adhesive were used to produce single lap joints. The static and fatigue strengths of single lap joints incorporating nanoparticles were compared to those without nanoparticles. Experiments were performed at 20 mm overlap length. DP460 epoxy was used as the adhesive material, and nano-Al2O3, nano-TiO2 and nano-SiO2 were used as the nanoparticles; and AISI 304 stainless steel plates were used as the adherents. The results of the experimental research revealed that average failure load increased significantly in nanoparticle-reinforced adhesive joints. The highest average failure load was obtained with 4 wt% nano-Al2O3 in epoxy adhesive. Fatigue tests were performed at 10 Hz frequency, and 0.1 loading ratio (R). When the fatigue test results were examined, it was observed that the addition of the nano-Al2O3 and nano-SiO2 to the adhesive increased fatigue strength of the adhesive joints, on the other hand, the addition of the nano-TiO2 to the adhesive reduced fatigue strength of the adhesive joints.