Βeta estradiol induces hormone-responsive behavior by regulating Akt-Sox9 signaling pathway in Hepg2 Cells
Medicine Science, cilt.15, sa.1, ss.89-93, 2026 (TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 15 Sayı: 1
- Basım Tarihi: 2026
- Doi Numarası: 10.5455/medscience.2025.07.202
- Dergi Adı: Medicine Science
- Derginin Tarandığı İndeksler: TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.89-93
- İnönü Üniversitesi Adresli: Evet
Özet
The varying rates of Hepatocellular carcinoma (HCC) incidence and mortality rates between genders highlight a critical need to understand how β-Estradiol (βEst) affects HCC cell lines. In order to reveal this, the proliferative and migratory characteristics and the protein expressions of the Akt-Sox9 pathway were evaluated in βEst-treated HepG2 HCC cells in vitro. Cell viability and cell migration characteristics, IL-6 levels measurement by ELISA, and Akt and Sox9 protein expression level measurement by Western blotting were performed in βEst-treated HepG2 cells. Results: In βEst-treated cells, migration rate (%/h) and overall migration rate (µm/h) were lowered to 0.5 and 2.6, respectively, and IL-6 levels were reduced to about 40 percent of that of control cells. In addition, the relative protein expression level of Akt (0.88-fold) and Sox9 (0.64-fold) was found to be downregulated following βEst treatment. According to the results of our in vitro study, HepG2 cell behaves as potentially hormone-responsive cells, and the results also support the clinical study findings about gender disparity in HCC.