Synthesis, structural characterizations and in vitro cytotoxic activities of new sulfonamide-based Schiff base derivatives

Sahal H., Oz S., TEKİN S., CANPOLAT E.

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1080/07391102.2022.2067237
  • Dergi Adı: JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Chemical Abstracts Core, EMBASE, MEDLINE
  • Anahtar Kelimeler: Sulfonamide derivative, Schiff base, spectroscopic, cytotoxic activity, cancer cell lines, DNA damage, RAPID COLORIMETRIC ASSAY, THERMAL CHARACTERIZATIONS, ANTIMICROBIAL ACTIVITY, ANTICANCER ACTIVITIES, CARBONIC-ANHYDRASE, INHIBITORS, GROWTH, SURVIVAL, SERIES
  • İnönü Üniversitesi Adresli: Evet

Özet

Synthesis of five different new compounds (1-5) were carried out. Their structures were characterized by spectroscopic methods such as Fourier transform infrared, Proton nuclear magnetic resonance, Carbon-13 nuclear magnetic resonance and Elemental analysis. Cytotoxic activities of five new sulfonamide based-Schiff base compounds were determined by MTT assay using A-2780 (human ovarian cancer) and MCF-7 (breast cancer) cell lines. LogIC(50) values of the sulfonamide derivates compounds were calculated by Graphpad Prism 12 programme after a 24-hour treatment for A2780 and MCF-7 cells. Comet assay experiments were performed to determine DNA damage using LogIC(50) concentrations of all compounds in A2780 and MCF-7 cells. All compounds significantly reduced A2780 and MCF-7 cell viability compared to control groups (p < 0.05). In addition, all compounds caused DNA damage in A2780 and MCF-7 cells (p < 0.05). These results show that the synthesized compounds exhibit cytotoxic effects against cancer cells and that the cause of cell death is due to DNA damage. Communicated by Ramaswamy H. Sarma