Total phenolic and flavonoid content of kumquat (Fortunella spp.) extracts prepared using different solvents


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Büyükkorkmaz Ç., Küçükbay F. , Hocagil M. M.

3rd International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2020), Ankara, Türkiye, 19 - 20 Mart 2020, ss.612

  • Basıldığı Şehir: Ankara
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.612

Özet

Cumquats or kumquats (Fortunella spp.) are a group of small fruit-bearing trees, belongs in the genus Fortunella of the family Rutaceae family. The fruits and leaves of Fortunella species have long been used in herbal medicine, especially for colds and coughs and other inflammation of the respiratory tract (1). It may be attributed to the presence of bioactive compounds such as flavonoids and phenolics in kumquat fruit. Since the health benefits of kumquat fruits were understood, its plantation and production have been increased both in Turkey and throughout the world in recent years. In fact, most of the kumquat literature published today focuses on the health benefits of the kumquat fruit. However, little information about the phytochemical constituents responsible for the observed activities. Thus in this study the fruits and leaves of kumquat and its six hybrids (EP.4, EP.29, EP.31, YP.117, YP.141, YP.188), obtained from Mersin Alata Horticultural Research Institute, were compared based on their total phenolics and flavonoids. In order to characterize the total phenolic and total flavonoid compounds in kumquat hybrids, extractions of kumquat fruit and leaf with different solvents, including ethanol and methanol of various concentrations and hexane were carried out and subjected to the analysis of total phenolic and flavonoid contents. Quantitative determination of total phenolics and flavonoids, of different solvent extracts were carried out using colorimetric methods (2,3). The total phenolic content, expressed as mg of gallic acid equivalent (GAE) per gram of extract, was found to be (3.705 - 20.281 mg GAE/g dry extract) and (31.062 - 86.329 mg GAE/g dry extract) for fruit and leaves respectively. The highest total phenolic content in leaf was observed in 60% methanol extract (86.329 mg GAE/g, YP188 hybrid). In the fruit of kumquat, the highest phenolic content was obtained in absolute methanol extracts (20.281 mg GAE/g, EP.4 hybrid). No significant difference could be observed among the extracts of wet and dry samples. The highest levels of total flavonoid were obtained in ethanol extract from leaves (632,222 mg QUE/g, EP.31). In the fruit of kumquat, the highest flavonoid content was obtained in absolute methanol extracts (313,333 mg QUE/g, YP.141).