Grapevines in central Anatolia region of Turkey were surveyed for the prevalence of grapevine leafroll viruses. The field study and collection of samples were conducted in nine major grapevine-growing areas. Samples collected from 622 vines were tested for Grapevine leafroll-associated virus 1, 2, 3 and 7 (GLRaV-1, -2, -3 and -7). According to diagnostic tests and surveys, 27 of 41 cultivars and 95 of 622 samples (15.27%) were found to be infected at least one virus. GLRaV-1 (8.36%) was found to be the most frequently encountered virus associated with leafroll disease of grapes, followed by GLRaV-3 (5.78%), GLRAV-7 (3.86%) and GLRAV-2 (2.41%).