Akademik Veri Yönetim Sistemi
Journal of Future Foods, cilt.6, sa.4, ss.589-603, 2026 (ESCI, Scopus)
Adjunct culture can be used to improve typical cheese aroma and contribute to proteolysis during ripening process. In the present study, some lactobacilli (Lactobacillus casei, L. delbrueckii subsp. bulgaricus, Lactiplantibacillus plantarum and two strains of L. helveticus) were tested in model cheeses during 120 days of ripening at the temperatures of 8 °C and 16 °C. It was aimed to investigate the effects of these parameters on free amino acid (FAA) content, volatile aroma profile and sensory properties in cheese. Volatile compounds in cheese samples were determined by gas chromatography/mass spectrometry (GC/MS) analysis and FAA content by high performance liquid chromatography (HPLC) analysis. Principal component (PCA), partial least squares-discriminant analysis (sPLSDA) and heat map cluster analysis were performed to distinguish different volatile aroma and FAAs in cheeses according to ripening time and temperature as well as adjunct culture. The predominant FAAs in the cheese were Arg, Tyr, Val, Leu, Trp and Lys, regardless of ripening. The highest concentration of Glu was observed in cheeses containing L. bulgaricus, followed by cheeses with L. helveticus and L. plantarum. A total of 41 volatile compounds were identified, with variations noted between the different cheeses in particular L. helveticus, play a crucial role in flavor development. The ripening temperature also influenced the production of volatile compounds: At 16 °C, cheeses with L. bulgaricus and L. helveticus were characterized by alcohols, esters and acids, while cheeses with L. plantarum contained mainly, acids, aldehydes and ketones. For actobacilli, especially combination of L. helveticus, L. bulgaricus and L. plantarum, can be added to the cheese to improve and intensify the flavor. It was seen that the positive effects of selected adjunct cultures on aroma and taste of model cheeses were related to volatiles and FAAs.