Magnetic-propelled Fe3O4-chitosan carriers enhance l-asparaginase catalytic activity: a promising strategy for enzyme immobilization


ATEŞ B. , ULU A., KÖYTEPE S. , ALI NOMA S. A. , KOLAT V. S. , İZGİ T.

RSC ADVANCES, cilt.8, ss.36063-36075, 2018 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 8 Konu: 63
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1039/c8ra06346j
  • Dergi Adı: RSC ADVANCES
  • Sayfa Sayıları: ss.36063-36075

Özet

Magnetic-propelled carriers comprising magnetic Fe3O4-chitosan nanoparticles were immobilized with l-asparaginase (l-ASNase). The enzyme displayed enhanced catalytic activity in a weak magnetic field, and thermal and pH stabilities. The conjugated l-ASNase presented higher thermostability and wider range of pH stability in comparison with those of free l-ASNase. Moreover, the reusability of conjugated l-ASNase significantly improved after immobilization and it retained 60.5% of its initial activity after undergoing 16 cycles. The conjugated l-ASNase maintained more than 50% and 48% initial activity after 4 weeks of storage at 4 degrees C and room temperature, respectively. Furthermore, we reveal that the activity of conjugated l-ASNase onto magnetic Fe3O4-chitosan particles increased by about 3-fold in the weak magnetic field at certain frequencies and flux density compared with that of free l-ASNase. Considering these excellent attributes, the magnetic-propelled mechanism in the transporting and activation of l-ASNase can be used by enhancing the catalytic activity, stability, and efficiency in vital implications for medicinal biotechnology.