ENHANCEMENT OF LACCASE PRODUCTION OF PRE-GROWN FUNGAL PELLETS IN WASTEWATER OF OLIVE OIL MILLS

APOHAN E., YEŞİLADA Ö.

FRESENIUS ENVIRONMENTAL BULLETIN, cilt.20, sa.5, ss.1216-1224, 2011 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 20 Sayı: 5
  • Basım Tarihi: 2011
  • Dergi Adı: FRESENIUS ENVIRONMENTAL BULLETIN
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1216-1224
  • Anahtar Kelimeler: Funalia trogii, Laccase enzyme, Olive oil mill wastewater, Repeated-batch, Trametes versicolor, MANGANESE PEROXIDASE, PLEUROTUS-OSTREATUS, DECOLORIZATION, TROGII, FERMENTATION, DETOXIFICATION, OPTIMIZATION, REMOVAL, LIQUID, ROLES
  • İnönü Üniversitesi Adresli: Evet

Özet

Previous works reported laccase production ability of batch cultures of white rot fungi in olive oil mill wastewater media (OOMW). Therefore, here, laccase production ability of repeated-batch cultures of Funalia trogii and Trametes versicolor pre-grown pellets in olive oil mill wastewater media (OOMW) was investigated and conditions for laccase production were optimized. The results indicated that high amounts of laccase could be obtained by using the best production conditions such as agitation, initial pellet amounts, initial pH, OOMW concentration and temperature. All these conditions were found to be important for laccase production. After optimization, the effect of various supplementary sources and inducer (glucose, malt extract, cheese whey and copper) on laccase production ability of these fungi and longevity of this production ability were examined in repeated-batch process. Presence of glucose, malt extract, cheese whey and copper positively affected the laccase production ability and longevity of the production. The fungal pellets also partially bioremediated the OOMW during laccase production and F. trogii pellets removed 33 % COD from OOMW effluent whilst T. versicolor removed 28 % in the first use.