Liver lipid peroxidation and antioxidant capacity in cerulein-induced acute pancreatitis

BATÇIOĞLU K., GÜL M., UYUMLU A. B., ESREFOGLU M.

BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH, cilt.42, sa.9, ss.776-782, 2009 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 42 Sayı: 9
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1590/s0100-879x2009000900001
  • Dergi Adı: BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.776-782
  • Anahtar Kelimeler: Acute pancreatitis, Liver injury, Oxidative damage, Pentoxifylline, L-NAME, L-arginine, FREE RADICAL PRODUCTION, NITRIC-OXIDE, OXIDATIVE STRESS, N-ACETYLCYSTEINE, ASCITIC FLUID, GLUTATHIONE, RAT, PENTOXIFYLLINE, PATHOGENESIS, ACTIVATION
  • İnönü Üniversitesi Adresli: Evet

Özet

The aim of this study was to evaluate the role of oxidative damage in pancreatitis-induced hepatic injury. Thirty-five rats were divided into five groups (each of 7 rats): control, cerulein (100 mu g/kg body weight), cerulein and pentoxifylline (12 mg/kg body weight), cerulein plus L-NAME (10 mg/kg body weight) and cerulein plus L-arginine (160 mg/kg body weight). The degree of hepatic cell degeneration differed significantly between groups. Mean malondialdehyde levels were 7.00 +/- 2.29, 20.89 +/- 10.13, 11.52 +/- 4.60, 18.69 +/- 8.56, and 8.58 +/- 3.68 nmol/mg protein for the control, cerulein, pentoxifylline, L-NAME, and L-arginine groups, respectively. Mean catalase activity was 3.20 +/- 0.83, 1.09 +/- 0.35, 2.05 +/- 0.91, 1.70 +/- 0.60, and 2.85 +/- 0.47 U/mg protein for the control, cerulein, pentoxifylline, L-NAME, and L-arginine groups, respectively, and mean glutathione peroxidase activity was 0.72 +/- 0.25, 0.33 +/- 0.09, 0.37 +/- 0.04, 0.34 +/- 0.07 and 0.42 +/- 0.1 U/mg protein for the control, cerulein, pentoxifylline, L-NAME, and L-arginine groups, respectively. Cerulein-induced liver damage was accompanied by a significant increase in tissue malondialdehyde levels (P < 0.05) and a significant decrease in catalase (P < 0.05) and GPx activities (P < 0.05). L-arginine and pentoxifylline, but not L-NAME, protected against this damage. Oxidative injury plays an important role not only in the pathogenesis of AP but also in pancreatitis-induced hepatic damage.