Abiotic stress is becoming more prevalent as the intensity of agriculture and the demand for farmable land are ever increasing. Besides drought and salinity stress, chilling or freezing stress is one of the most important limiting factors of crop production all around the world. Salicylic acid (SA) is a common plant-produced signal molecule that is responsible for inducing resistance to a number of biotic and abiotic stresses. This study was conducted to determine the effect of foliar SA applications on fruit-quality characteristics and yield of strawberry under antifrost heated greenhouse conditions in two successive experiments. Spraying of 1 mM SA (1 mM) was done once (SA1), twice (SA2), three times (SA3), or four times (SA4) during the vegetation period with 7 d intervals. Concentrations of chlorophyll and minerals were measured in leaves, and vitamin C (ascorbic acid), total soluble solids (TSS), titratable acidity (TA), and color (a*) in fruits. Fruit weight, early yield, and total yield were also determined. Foliar applications of SA positively affected TSS and ascorbic acid (AA) content of strawberry fruits. Salicylic acid treatments had no effect on TA of strawberry fruits. SA4 treatment gave the greatest a* in fruit and chlorophyll-concentration values in the leaves. It was shown that SA treatments increased the content of all nutrients in the leaves of strawberry plants, and greater values often were obtained from SA3 and SA4 treatments. The early yield and total yield of strawberry were significantly affected by SA applications, among which SA3 and SA4 resulted in the highest early and total yields. The present study suggests that SA3 and SA4 treatments can ameliorate the deleterious effects of low temperatures on strawberry plants and that SA application may offer an economical and simple method for low-temperature protection.