Fabrication, thermal and in vitro behaviors of ciprofloxacin loaded beta-cyclodextrin-PEG based polyurethanes as potential biomaterial for wound dressing applications


Kantarcioglu M., Tunc M. G. K. , GÜRSES C., ATEŞ B., KÖYTEPE S.

JOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRY, 2022 (Peer-Reviewed Journal) identifier

  • Publication Type: Article / Article
  • Publication Date: 2022
  • Doi Number: 10.1080/10601325.2022.2048954
  • Journal Name: JOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRY
  • Journal Indexes: Science Citation Index Expanded, Scopus, Academic Search Premier, PASCAL, Aerospace Database, Biotechnology Research Abstracts, CAB Abstracts, Chimica, Communication Abstracts, Compendex, Metadex, Civil Engineering Abstracts
  • Keywords: Polyurethane, beta-cyclodextrin, ciprofloxacin, antibacterial film, biocompatibility, POLYMERIC MATERIALS, DEXTRIN, HYDROGEL, PLASTICS, COMPLEXATION, DELIVERY, RELEASE

Abstract

Antibacterial polyurethanes as wound-dressing material with high biocompatibility and thermal stability were developed from polyethylene glycol-200 (PEG200), 4,4'-methylenebis(cyclohexyl isocyanate) (HMDI) and beta-cyclodextrin (beta CD). beta CD was preferred in this material because of providing biocompatibility and supporting the absorption/desorption characteristics of ciprofloxacin due to its hydrophobic gap. The beta-cyclodextrin-PEG based polyurethane structures (PU-200-beta CDs) was synthesized with different beta-cyclodextrin:PEG:HMDI ratios. PU-200-beta CDs were characterized by FTIR spectroscopy, thermal analysis, SEM, and water contact angle techniques. The biocompatibility property of polyurethane materials was determined according to the indirect cytotoxicity assay results and the PU-200-beta CDs exhibited cell viability ranging from, 83.04 +/- 7.28% to 99.73 +/- 10.3% against L-929 cells. The hydrolytic degradability test was applied to the highest biocompatible PU-200-beta CD3 structure. The mass loss of PU-200-beta CD3 was determined 4.75 +/- 0.86% at the end of the 28-day. Antibacterial properties of ciprofloxacin doped PU-200-beta CD3 were investigated using Escherichia coli and Bacillus subtilis. The ideal polyurethane structure was selected according to its biocompatibility and antibacterial properties. This polyurethane structure was converted into wound dressing material via electrospinning technique. The obtained dressing material showed mechanical stable fiber structure. Therefore, prepared beta-cyclodextrin-PEG-based polyurethane structure can be suitable for the production of wound-dressing material due to its good ciprofloxacin release properties, high stability and biocompatibility.