Antitumor Effects of Horsetail Extract in Hepatocellular Cancer Cell Line SNU-449

Uyumlu A. B.

8 th International Hippocrates Congress on Medical and Health Sciences, Gaziantep, Türkiye, 4 - 05 Mart 2022, ss.6-7

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Gaziantep
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.6-7
  • İnönü Üniversitesi Adresli: Evet

Özet

Abstract Aim-Background: Natural products used against cancer are becoming important for treatment. Horsetail (Equisetum arvense) extract (HTE) is used in traditional medicine to treat various diseases such as tuberculosis, kidney disorders and bladder disease. The plant contains alkaloids, phytosterols, saponins, ascorbic acid, silicic acid, tannin, and triterpenoids. The role of HTE as an antitumor agent in high-grade hepatocellular cancer is unknown. The aim of the present study is to evaluate the antineoplastic efficacy of HTE in HCC cell line SNU-449. Materials and methods: Commercial plant extracts (Solgar-U.S.A) obtained from Equisetum arvense were used. To evaluate the antiproliferative and antimetastatic effects of HTE in HCC, cell viability (MTT) assay was performed at 24, 48, and 72nd hour intervals and at seven different concentrations (between 7.81-500 ppm) of HTE. The minimum effective concentration on cell viability was chosen and later three procedures were performed at the designated dose at 24th hour of exposure to HTE. In addition to MTT assay, colony formation, wound healing assays, and western blotting for Caspase-3 and Cleaved Caspase-3 were performed. Results: MTT assay showed that 326 ppm at 24 hours was the effective minimum dose. Absorbance in 326 ppm and HTE-treated and untreated groups were 0.316 (0.234-0.388) and 0.615 (0.481-0.759); respectively. Colony formation assay showed that there was a significant difference between HTE -treated and untreated cells (46,9% surviving fraction relative to control). Wound closure rate was 43,4% in HTE - treated cells at 24th hour. Normalized volume ratios for Caspase-3 were 53222328 and 7948593 in HTE -treated and control cells, respectively. Normalized volume ratios for Cleaved Caspase-3 were 707454 and 596409 in HTE -treated and control cells, respectively. Conclusions: Our study revealed that HTE possessed an antiproliferative and antimetastatic effects against SNU-449 cells. Further study of the mechanism of action of HTE is needed.